71376

Sigma-Aldrich

Sodium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym(s):
Halite, NaCl
Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein/REAXYS Number:
3534976
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

product line

BioUltra

assay

≥99.5% (AT)

form

solid

availability

not available in (Sales restrictions may apply)

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

801 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

bromide (Br-): ≤50 mg/kg
hexacyanoferrate(II) ([Fe(CN)6]4-): ≤1 mg/kg
iodide (I-): ≤10 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

SMILES string

[Na+].[Cl-]

UV absorption

λ: 260 nm Amax: ≤0.01
λ: 280 nm Amax: ≤0.01

suitability

suitable for molecular biology

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Application

Sodium chloride has been used:
  • as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry
  • in the preparation of Bold′s basal medium (BBM)
  • as a component of transcription and annealing buffer

Biochem/physiol Actions

Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.

Other Notes

Easily compare specifications for Sodium chloride products with the Sodium chloride specification table.
Sales restrictions may apply
Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA; Simple salting out procedure for extracting DNA from human nucleated cells

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria (Peltigerales, Ascomycota)
Cornejo C, et al.
Bio-protocol (2015)
A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor
Kim T, et al.
Sensors, 15(3), 6469-6482 (2015)
Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells
Kleinewietfeld M, et al.
Nature, 496(7446), 518-518 (2013)
A simple salting out procedure for extracting DNA from human nucleated cells.
S A Miller et al.
Nucleic acids research, 16(3), 1215-1215 (1988-02-11)
D.M. Wallace
Methods in Enzymology, 152, 39-39 (1987)

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