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Sigma-Aldrich

NBT-BCIP® solution

BioReagent, suitable as substrate for alkaline phosphatase in dot blots

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Synonym(s):
BCIP®/NBT, NBT-X-phosphate
UNSPSC Code:
12171500
PubChem Substance ID:
329763805
NACRES:
NA.21

grade

suitable as substrate for alkaline phosphatase in dot blots

product line

BioReagent

form

liquid

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChI key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

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Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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Makrina Totsika et al.
Journal of bacteriology, 191(12), 3901-3908 (2009-04-21)
Disulfide bond (DSB) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. Uropathogenic Escherichia coli (UPEC) strains possess two paralogous disulfide bond systems: the well-characterized DsbAB system
Ludovic Sauguet et al.
Nucleic acids research, 39(10), 4475-4489 (2011-02-08)
Cyclodipeptide synthases (CDPSs) belong to a newly defined family of enzymes that use aminoacyl-tRNAs (aa-tRNAs) as substrates to synthesize the two peptide bonds of various cyclodipeptides, which are the precursors of many natural products with noteworthy biological activities. Here, we
D Thompson et al.
BioTechniques, 12(5), 656-658 (1992-05-01)
A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels
Andrea L H Arnett et al.
Frontiers in microbiology, 2, 220-220 (2011-11-09)
Recombinant adeno-associated viral (rAAV) vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for
Olga Turovskaya et al.
Carcinogenesis, 29(10), 2035-2043 (2008-08-12)
Patients with inflammatory bowel diseases are at increased risk for colorectal cancer, but the molecular mechanisms linking inflammation and cancer are not well defined. We earlier showed that carboxylated N-glycans expressed on receptor for advanced glycation end products (RAGE) and
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