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MilliporeSigma

72091

Sigma-Aldrich

NBT-BCIP® solution

BioReagent

Synonym(s):

BCIP®/NBT, NBT-X-phosphate

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2.5 ML
$106.00
10 ML
$331.00

$106.00

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
329763805
NACRES:
NA.21

Key Documents

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product line

BioReagent

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

Inchi Key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

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Show Differences

1 of 4

This Item
B19111168145100194046
NBT-BCIP® solution BioReagent

Sigma-Aldrich

72091

NBT-BCIP® solution

BCIP®/NBT Liquid Substrate System ready to use solution

Sigma-Aldrich

B1911

BCIP®/NBT Liquid Substrate System

NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

Sodium phosphate dibasic solution BioUltra, 0.5 M in H2O

Sigma-Aldrich

94046

Sodium phosphate dibasic solution

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

suitable as substrate for alkaline phosphatase in dot blots

grade

-

grade

-

grade

-

form

liquid

form

liquid

form

solution

form

liquid

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

solubility

-

solubility

-

solubility

H2O: 0.5 M at 20 °C, clear, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity[1]

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

152.6 °F - closed cup

flash_point_c

67 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8209
BCBV6055
BCBS2526V
BCBP6799V
BCBN5576V

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Ludovic Sauguet et al.
Nucleic acids research, 39(10), 4475-4489 (2011-02-08)
Cyclodipeptide synthases (CDPSs) belong to a newly defined family of enzymes that use aminoacyl-tRNAs (aa-tRNAs) as substrates to synthesize the two peptide bonds of various cyclodipeptides, which are the precursors of many natural products with noteworthy biological activities. Here, we
Ying Wu et al.
Infection and immunity, 76(5), 2249-2255 (2008-03-12)
The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted
D Thompson et al.
BioTechniques, 12(5), 656-658 (1992-05-01)
A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels
Andrea L H Arnett et al.
Frontiers in microbiology, 2, 220-220 (2011-11-09)
Recombinant adeno-associated viral (rAAV) vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for
Makrina Totsika et al.
Journal of bacteriology, 191(12), 3901-3908 (2009-04-21)
Disulfide bond (DSB) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. Uropathogenic Escherichia coli (UPEC) strains possess two paralogous disulfide bond systems: the well-characterized DsbAB system
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