MilliporeSigma
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72464

Supelco

Atto 565 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Empirical Formula (Hill Notation):
C35H34ClN3O11
Molecular Weight:
708.11
MDL number:
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥90% (HPLC)
≥90% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

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General description

Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.

Features and Benefits

  • Strong absorption
  • High fluorescence quantum yield
  • High thermal and photo-stability

Other Notes

Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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