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73144

Sigma-Aldrich

Resorufin

95%

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Synonym(s):
7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
Beilstein/REAXYS Number:
174850
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥95.0% (HPLC)
95%

form

solid

mp

>300 °C (lit.)

storage temp.

−20°C

SMILES string

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H

InChI key

HSSLDCABUXLXKM-UHFFFAOYSA-N

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This Item
R4883R4758E3763
Resorufin 95%

73144

Resorufin

Resorufin β-D-galactopyranoside ~95%

R4883

Resorufin β-D-galactopyranoside

Resorufin β-D-glucopyranoside ≥90%

R4758

Resorufin β-D-glucopyranoside

Resorufin ethyl ether ≥98% (TLC), powder

E3763

Resorufin ethyl ether

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥95.0% (HPLC), 95%

assay

~95%

assay

≥90%

assay

≥98% (TLC)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

mp

>300 °C (lit.)

mp

-

mp

-

mp

223-225 °C (lit.)

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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