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73144

Sigma-Aldrich

Resorufin

95%

Synonym(s):
7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
Beilstein:
174850
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Assay

≥95.0% (HPLC)
95%

form

solid

mp

>300 °C (lit.)

storage temp.

−20°C

SMILES string

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H

InChI key

HSSLDCABUXLXKM-UHFFFAOYSA-N

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This Item
W358908307521SMB00428
Resorufin 95%

Sigma-Aldrich

73144

Resorufin

Resorcinol ≥98%, FG

Sigma-Aldrich

W358908

Resorcinol

Resorcinol ReagentPlus®, 99%

Sigma-Aldrich

307521

Resorcinol

Resibufogenin ≥98% (HPLC)

Sigma-Aldrich

SMB00428

Resibufogenin

assay

≥95.0% (HPLC), 95%

assay

≥98%

assay

99%

assay

≥98% (HPLC)

form

solid

form

flakes

form

chips, powder or granules

form

powder

mp

>300 °C (lit.)

mp

109-112 °C (lit.)

mp

109-112 °C (lit.)

mp

-

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

100

Quality Level

400

Quality Level

200

Quality Level

200

General description

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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