Atto 655 NHS ester

BioReagent, suitable for fluorescence

MDL number:
PubChem Substance ID:

Quality Level

product line



λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0


suitable for coupling to amines
suitable for fluorescence

storage temp.




InChI key


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Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Gerald Giller et al.
Nucleic acids research, 31(10), 2630-2635 (2003-05-09)
Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were...
Ye Chen et al.
Biomedical optics express, 10(3), 1257-1272 (2019-03-21)
Open-top light-sheet microscopy is a technique that can potentially enable rapid ex vivo inspection of large tissue surfaces and volumes. Here, we have optimized an open-top light-sheet (OTLS) microscope and image-processing workflow for the comprehensive examination of surgical margin surfaces...
Highly sensitive protease assay using fluorescence quenching of peptide probes based on photoinduced electron transfer.
Nicole Marmé et al.
Angewandte Chemie (International ed. in English), 43(29), 3798-3801 (2004-07-20)
Taurai Tasara et al.
Nucleic acids research, 31(10), 2636-2646 (2003-05-09)
The modification of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here...
Nicole Marmé et al.
Bioconjugate chemistry, 14(6), 1133-1139 (2003-11-20)
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655...
Fluorescent Labeling of Peptides
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Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies
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