76508

Sigma-Aldrich

Atto 647N Protein Labeling Kit

NACRES:
NA.32

Quality Level

fluorescence

λex 647 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

storage temp.

2-8°C

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General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Alexandra Grubman et al.
Nature communications, 12(1), 3015-3015 (2021-05-23)
The role of microglia cells in Alzheimer's disease (AD) is well recognized, however their molecular and functional diversity remain unclear. Here, we isolated amyloid plaque-containing (using labelling with methoxy-XO4, XO4+) and non-containing (XO4-) microglia from an AD mouse model. Transcriptomics...
Bryant L Doss et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(23), 12817-12825 (2020-05-24)
Morphogenesis, tumor formation, and wound healing are regulated by tissue rigidity. Focal adhesion behavior is locally regulated by stiffness; however, how cells globally adapt, detect, and respond to rigidity remains unknown. Here, we studied the interplay between the rheological properties...
Shiqiong Hu et al.
Molecular biology of the cell, 30(16), 2025-2036 (2019-06-20)
Contractile arrays of actin and myosin II filaments drive many essential processes in nonmuscle cells, including migration and adhesion. Sequential organization of actin and myosin along one dimension is followed by expansion into a two-dimensional network of parallel actomyosin fibers...
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
Articles
We offer protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes. Both series of kits provide an easy and reliable way to fluorescently label purified proteins, enzymes, and antibodies.
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