Skip to Content
MilliporeSigma
All Photos(1)

Documents

77617

Sigma-Aldrich

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352401
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

technique(s)

ChIP: suitable
DNA extraction: suitable
DNA purification: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

7.2-8.3(H2O-phase, after extraction with H2O, 1:1)

density

1.28 g/mL at 20 °C

absorption

cut-off at 360 nm

suitability

suitable for molecular biology

application(s)

agriculture

storage temp.

2-8°C

SMILES string

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Phenol-chloroform-isoamyl alcohol mixture (PCI-mixture) is a widely utilized mixture in molecular biology and biochemical research, specifically designed for nucleic acid purification, including DNA and RNA. With its essential composition of 25% phenol, 24% chloroform, and 1% isoamyl alcohol, PCI-mixture plays a pivotal role in separating proteins from nucleic acids during the purification process. Phenol denatures proteins, chloroform creates a distinct layer to trap them, and isoamyl alcohol facilitates the clear separation of these layers, enabling the isolation of purified DNA or RNA in the aqueous phase.
The applications of PCI-mixture are diverse, encompassing RNA isolation, where it efficiently extracts RNA from animal and plant tissues for downstream analyses like reverse transcription and gene expression studies. Further, PCI-mixture also proves instrumental in DNA extraction, enabling efficient recovery for various applications such as DNA minisatellite fingerprinting for genetic identification. As a general purification technique, PCI-mixture ensures high-quality preparations of both DNA and RNA for further research.

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.Further, also in the extraction of DNA for the generation of DNA minisatellite fingerprints

Biochem/physiol Actions

When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Free from DNase, RNase, NICKase and protease

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

For additional information on our range of Biochemicals, please complete this form.

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

174.2 °F

flash_point_c

79 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A genetic program mediates cold-warming response and promotes stress-induced phenoptosis in C. elegans
Jiang W, et al.
eLife, 7, e35037-e35037 (2018)
E J Wickings
Electrophoresis, 16(9), 1678-1683 (1995-09-01)
DNA fingerprints using three oligonucleotide probes, a (GTG)5 short tandem repeat sequence, and 2 corresponding to the core sequences of minisatellites 33.15 and 33.6, have been used to generate DNA fingerprints of a captive colony of mandrills (Mandrillus sphinx) housed
Peribiliary glands are key in regeneration of the human biliary epithelium after severe bile duct injury
de Jong IEM, et al.
Hepatology (2018)
Extensive mitochondrial population structure and haplotype-specific phenotypic variation in the Drosophila Genetic
Bevers RPJ, et al.
bioRxiv, 466771-466771 (2018)
Octopaminergic neurons have multiple targets in Drosophila larval mushroom body calyx and regulate behavioral odor discrimination
McLachlan AD, et al.
bioRxiv, 295659-295659 (2018)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service