77630

Sigma-Aldrich

Phenolphthalein bisphosphate pyridine salt

≥90% (T)

Synonym(s):
Phenolphthalein diphosphate pyridine salt
Empirical Formula (Hill Notation):
C20H16O10P2 · C5H5N
CAS Number:
Molecular Weight:
557.38
MDL number:
PubChem Substance ID:
NACRES:
NA.83
Pricing and availability is not currently available.

Quality Level

assay

≥90% (T)

form

powder

impurities

≤0.05% free phenolphthalein

loss

≤2% loss on drying

storage temp.

2-8°C

SMILES string

c1ccncc1.OP(O)(=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(OP(O)(O)=O)cc5

InChI

1S/C20H16O10P2.C5H5N/c21-19-17-3-1-2-4-18(17)20(28-19,13-5-9-15(10-6-13)29-31(22,23)24)14-7-11-16(12-8-14)30-32(25,26)27;1-2-4-6-5-3-1/h1-12H,(H2,22,23,24)(H2,25,26,27);1-5H

InChI key

DBXHFBVMRQOQKW-UHFFFAOYSA-N

Application

Suitable as substrate for phosphatase.

Other Notes

Chromogenic substrate for acid and alkaline phosphatases

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Changes in the phosphatase activity of Baker's yeast during the growth phase and location of the phosphatases in the yeast cell.
H SUOMALAINEN et al.
Biochimica et biophysica acta, 37, 482-490 (1960-01-29)
One-step automated alkaline phosphatase analysis.
D Comfort et al.
Clinica chimica acta; international journal of clinical chemistry, 14(2), 263-269 (1966-08-01)
J J Thrasher et al.
Journal - Association of Official Analytical Chemists, 64(1), 196-198 (1981-01-01)
A chemical method was developed to determine the presence of mammalian feces in food. The method consisted of the colorimetric determination of alkaline phosphatase and involved the splitting of the phosphate radical and phenolphthalein diphosphate and the resultant color development...
A simple and reliable method for estimation of alkaline phosphatase in tissue homogenates.
J P Manning et al.
Enzymologia, 31(5), 309-320 (1966-11-30)
Microdetermination of phosphatases employing phenolphthalein diphosphate as substrate.
J Fischl et al.
Clinical chemistry, 13(11), 941-946 (1967-11-01)

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