81188

Sigma-Aldrich

Poly(ethylene glycol)

BioUltra, 1,000

Synonym(s):
PEG
Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

Quality Level

product line

BioUltra

mol wt

Mr 950-1050

impurities

insoluble matter, passes filter test

pH

5.5-7.0 (25 °C, 50 mg/mL in H2O)

solubility

H2O: 50 mg/mL at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤50 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

50 mg/mL in H2O

UV absorption

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.05

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Application

Poly(ethylene glycol) has been used in the transformation mix solution of yeast. It has been used to prepare an agar-solute substrate to study the influence of cocoon of Cyclorana australis on liquid water exchange with the substrate.

Other Notes

In protein crystallization; As fusogen to obtain hybridomas for monoclonal antibody production; Significantly enhances the transfer of membrane immunoblotting

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

L. Olsson et al.
Methods in Enzymology, 92, 10-10 (1983)
C Zeng et al.
Analytical biochemistry, 189(2), 197-201 (1990-09-01)
Poly(ethylene glycol)n is a group of water-soluble, hydrophobic, optically transparent and biomacromolecule-nondenaturing polymers. These properties have caused it be widely used for various purposes in the biological sciences. In this study, the effects of poly(ethylene glycol)n on protein preservation, electrotransferring...
The cocoon of the fossorial frog Cyclorana australis functions primarily as a barrier to water exchange with the substrate.
Reynolds SJ, et al.
Physiological and Biochemical Zoology : Pbz, 83, 877-877 (2010)
Nutrient supplements boost yeast transformation efficiency.
Yu SC, et al.
Scientific Reports, 6, 35738-35738 (2016)
Protein crystallization: the growth of large-scale single crystals.
G L Gilliland et al.
Methods in enzymology, 104, 370-381 (1984-01-01)

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