81268

Sigma-Aldrich

Poly(ethylene glycol)

BioUltra, for molecular biology, 8,000

Synonym(s):
PEG
Linear Formula:
H(OCH2CH2)nOH
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

Quality Level

grade

for molecular biology

product line

BioUltra

form

powder

mol wt

Mr 7000-9000

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5-7.0 (25 °C, 50 mg/mL in H2O)

mp

59-64 °C

solubility

H2O: 50 mg/mL at 20 °C, clear, colorless

density

1.0845 g/mL at 70 °C
1.0689 g/mL at 90 °C

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

50 mg/mL in H2O

UV absorption

λ: 260 nm Amax: 0.15
λ: 280 nm Amax: 0.10

SMILES string

C(CO)O

storage temp.

2-8°C

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Application

Poly(ethylene glycol) has been used as a virioplankton concentrating agent. It has been used in the procedure involving ligation of 5′ mRNAs to RNA oligo linker.

Other Notes

Precipitant in the large-scale preparation of λgt10 bacteriophage

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Unexpected and novel putative viruses in the sediments of a deep-dark permanently anoxic freshwater habitat.
Borrel G, et al.
The ISME Journal, 6, 2119-2127 (2012)
Robust analysis of 5'-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sites.
Gowda M, et al.
Nature Protocols, 2, 1622-1632 (2007)
Cloning cDNA into lambda gt10 and lambda gt11.
J Jendrisak et al.
Methods in enzymology, 152, 359-371 (1987-01-01)
Virioplankton 'pegylation': use of PEG (polyethylene glycol) to concentrate and purify viruses in pelagic ecosystems.
Colombet J, et al.
Journal of Microbiological Methods, 71, 212-219 (2007)
Isolation and characterization of GoDREB encoding an ERF-type protein in forage legume Galegae orientalis.
Wang X, et al.
Genes & Genetic Systems, 85, 157-166 (2010)

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