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818M-9

Sigma-Aldrich

Cytokeratin 8 & 18 (B22.1 & B23.1) Mouse Monoclonal Antibody

NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

diluted ascites fluid

antibody product type

primary antibodies

clone

B22.1 & B23.1, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (818M-94)
vial of 0.5 mL concentrate (818M-95)
bottle of 1.0 mL predilute (818M-97)
vial of 1.0 mL concentrate (818M-96)
bottle of 7.0 mL predilute (818M-98)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotype

IgG1κ

control

prostate, salivary gland

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

Gene Information

human ... KRT8(3856)

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This Item
905H-0MABT844SAB4200855
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

diluted ascites fluid

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified from hybridoma cell culture

clone

B22.1 & B23.1, monoclonal

clone

EP1601Y + LL002, monoclonal

clone

L2A1, monoclonal

clone

CY-90, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

liquid

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

General description

Cytokeratins 8 &18 (CK 8 & 18) are major components of the intermediate filaments in the thyroid, liver, and most simple epithelia (e.g. breast, gastrointestinal and respiratory tracts). Anti-CK 8 & 18 is found to stain most adenocarcinomas and squamous cell carcinomas. CK8 & 18 are frequently used as the epithelial markers for identifying Paget cells, metastatic gastric, and colorectal carcinomas.

Quality


IVD

IVD

IVD

RUO

Linkage

Cytokeratin 8 & 18 Positive Control Slides, Product No. 818S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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J M Corson
Pathology annual, 21 Pt 2, 47-81 (1986-01-01)
Keratin protein immunohistochemistry is a powerful diagnostic tool whose role has already been firmly established in many surgical pathology laboratories. Recent studies of the biology of keratin proteins have defined the heterogeneity of keratin protein expression among various epithelial tissues
B Angus et al.
The Journal of pathology, 153(4), 377-384 (1987-12-01)
Production of a new monoclonal antibody designated NCL-5D3 is described. The antibody recognizes several low molecular weight cytokeratins, in particular cytokeratin Moll number 8 as determined by immunoblotting studies, and is highly effective for immunocytochemistry using routinely processed paraffin-embedded material.
M Sasaki et al.
Histopathology, 32(3), 199-208 (1998-05-06)
Recent reports suggest that genetic examination of K-ras or p53 mutation is more sensitive for the detection of occult lymph node metastasis in colorectal carcinomas than conventional examination by haematoxylin and eosin (H & E) staining or immunohistochemistry for gene

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