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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

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Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

concentration

1 M in H2O
238 g/L HEPES in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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1 of 4

This Item
H3537H2383054457
vibrant-m

83264

HEPES buffer solution

-
vibrant-m

H3537

HEPES solution

Premium Grade
vibrant-m

H23830

HEPES

Essential Grade
vibrant-m

54457

HEPES

Premium Grade
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

crystalline

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

pH

5.5±0.5

pH

5.0-6.0

pH

-

pH

5.0-6.5 (25 °C, 1 M in H2O)

density

1.07 g/mL at 20 °C

density

-

density

-

density

-

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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