MilliporeSigma
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83689

Sigma-Aldrich

Rhodamine B

for fluorescence

Synonym(s):
Rhodamine O, Brilliant Pink B, Basic Violet 10, Tetraethylrhodamine
Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
Molecular Weight:
479.01
Colour Index Number:
45170
Beilstein:
4091619
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

grade

for fluorescence

form

powder or crystals

mp

210-211 (dec.) (lit.)

solubility

H2O: soluble
methanol: soluble

fluorescence

λex 553 nm; λem 627 nm in methanol

suitability

in accordance for fluorescence

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

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General description

Rhodamine B is bright-red in color.
Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.

Application

Rhodamine B has been used:
  • as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
  • as a fluorescent dye in confocal microscopy
  • to study its fluorescence in water and ethanol solutions

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)

Analysis Note

λmax. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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