83689

Rhodamine B

suitable for fluorescence

• Synonym(s): Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine
• Empirical Formula (Hill Notation): C₂₈H₃₁ClN₂O₃
• CAS Number: 81-88-9
• Molecular Weight: 479.01
• Colour Index Number: 45170
• Beilstein/REAXYS Number: 4091619
• EC Number: 201-383-9
• MDL number: MFCD00011931
• UNSPSC Code: 12171500
• PubChem Substance ID: 57652962
• NACRES: NA.32

Properties

• form: powder or crystals
• Quality Level: 200
• mol wt: 479.01 g/mol
• concentration: 80-100 % (w/w) (Rhodamine B)
• color: green to very dark green
• mp: 210-211 (dec.) (lit.)
• solubility: H₂O: soluble; methanol: soluble
• density: 0.79 g/cm³
• fluorescence: λ_ex 553 nm; λ_em 627 nm in methanol
• suitability: in accordance for fluorescence; suitable for fluorescence
• SMILES string: [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1
• InChI: 1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H
• InChI key: PYWVYCXTNDRMGF-UHFFFAOYSA-N

Description

General description

Rhodamine B is a xanthene dye,[1] which functions as a water tracer fluorescent.[2] It is used as a staining fluorescent dye.[3]

Rhodamine B is bright-red in color.[4]

Application

Rhodamine B has been used:

• as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)[5]
• as a fluorescent dye in confocal microscopy[6]
• to study its fluorescence in water and ethanol solutions[7]

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)[5]

Analysis Note

λ_max. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H318,H412
• pcodes: P264 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves