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83695

Sigma-Aldrich

Rhodamine 110 chloride

suitable for fluorescence, BioReagent, ≥99.0% (UV)

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Empirical Formula (Hill Notation):
C20H14N2O3 · HCl
CAS Number:
Molecular Weight:
366.80
Beilstein:
4631860
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Assay

≥99.0% (UV)

form

crystals

mp

>300 °C (lit.)

solubility

ethanol: soluble
methanol: soluble

fluorescence

λex 498 nm; λem 520 nm in methanol

suitability

suitable for fluorescence

SMILES string

Cl.Nc1ccc2c(OC3=CC(=N)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H

InChI key

JNGRENQDBKMCCR-UHFFFAOYSA-N

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This Item
43220283689R1755
Rhodamine 110 chloride suitable for fluorescence, BioReagent, ≥99.0% (UV)

Sigma-Aldrich

83695

Rhodamine 110 chloride

Rhodamine B for fluorescence

Sigma-Aldrich

83689

Rhodamine B

assay

≥99.0% (UV)

assay

-

assay

-

assay

-

form

crystals

form

powder or crystals

form

powder or crystals

form

-

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

210-211 (dec.) (lit.)

mp

-

solubility

ethanol: soluble, methanol: soluble

solubility

-

solubility

H2O: soluble, methanol: soluble

solubility

-

fluorescence

λex 498 nm; λem 520 nm in methanol

fluorescence

-

fluorescence

λex 553 nm; λem 627 nm in methanol

fluorescence

λex 543 nm; λem 580 nm in methanol

General description

Rhodamine 110 or R110 is a triphenylmethane dye and emits a strong green-fluorescent light. The high fluorescence is attributed to the presence of amino groups as electron donating groups. Rhodamine 110 has excitation and emission maximum at 502.4/521.6 nm, respectively.

Application

Rhodamine 110 is used for the determination of protease activity with accurate kinetic parameters. It is used to measure caspase activity in cells with induced apoptosis.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Customers Also Viewed

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Xian Zhang et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 59(8), 1667-1672 (2003-05-09)
A simple and sensitive fluorescence quenching method for the determination of trace nitrite has been developed. The method is based on the reaction of Rhodamine 110 with nitrite in acidic medium to form a new compound, which has much lower
Yan Liang et al.
Journal of the American Chemical Society, 132(18), 6306-6308 (2010-04-20)
Access to native protein structure depends on precise polypeptide folding and assembly pathways. Identifying folding missteps that may lead to the nearly 40 protein misfolding diseases could feature prominently in the development of intervention strategies. Accordingly, we have investigated the
Marisa Ninivaggi et al.
Clinical chemistry, 58(8), 1252-1259 (2012-06-06)
The calibrated automated thrombogram (CAT) assay in plasma is a versatile tool to investigate patients with hypo- or hypercoagulable phenotypes. The objective was to make this method applicable for whole blood measurements. Thin-layer technology and the use of a rhodamine
Tatyana G Terentyeva et al.
Bioconjugate chemistry, 22(10), 1932-1938 (2011-09-13)
Commonly used fluorogenic substrate analogues for the detection of protease activity contain two enzyme-cleavable bonds conjugated to the fluorophore. Enzymatic cleavage follows a two-step reaction with a monoamide intermediate. This intermediate shows fluorescence at the same wavelength as the final
Ana Virel et al.
Analytical chemistry, 84(5), 2380-2387 (2012-02-14)
By site directed mutagenesis, we have produced recombinant mutants of human and mouse prethrombin-2 which are able to convert themselves autocatalytically into α-thrombin. We also have created a new method to amplify the signal of bioanalytical assays based on the

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