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83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

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Synonym(s):
2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
Linear Formula:
C21H17ClN2O3
CAS Number:
Molecular Weight:
380.82
Beilstein:
6030951
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for fluorescence

Quality Level

product line

BioReagent

Assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

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This Item
8368983695R8881
Rhodamine 123 BioReagent, for fluorescence, ≥85% (HPLC)

Sigma-Aldrich

83702

Rhodamine 123

Rhodamine B for fluorescence

Sigma-Aldrich

83689

Rhodamine B

Rhodamine 110 chloride suitable for fluorescence, BioReagent, ≥99.0% (UV)

Sigma-Aldrich

83695

Rhodamine 110 chloride

product line

BioReagent

product line

-

product line

BioReagent

product line

-

assay

≥85% (HPLC)

assay

-

assay

≥99.0% (UV)

assay

-

form

powder

form

powder or crystals

form

crystals

form

powder

solubility

ethanol: 20 mg/mL, clear, red

solubility

H2O: soluble, methanol: soluble

solubility

ethanol: soluble, methanol: soluble

solubility

water: 10 mg/mL, clear, pink

suitability

in accordance for fluorescence

suitability

in accordance for fluorescence

suitability

-

suitability

-

General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
  • to label the cells for estimating the P-gp expression by flow cytometry
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
  • the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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