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Sigma-Aldrich

Schiff′s reagent for aldehydes

for microscopy

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Synonym(s):
Fuchsin sulfurous acid
NACRES:
NA.21

grade

for microscopy

form

liquid

application(s)

food and beverages

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This Item
B3928S513371115
form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

application(s)

food and beverages

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

General description

Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.

Application

Schiff′s reagent for aldehydes has been used:
  • in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water
  • to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants
  • in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Met. Corr. 1

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
David R Jackson et al.
ACS chemical biology, 16(1), 6-13 (2020-12-23)
Plasmalogens are vinyl ether-containing lipids produced by mammals and bacteria. The aerobic biosynthetic pathway in eukaryotes and bacteria is known, but the anaerobic pathway has remained a mystery. Here, we describe a two-gene operon (plasmalogen synthase, pls) responsible for plasmalogen
Paul Breen et al.
Infection and immunity, 89(9), e0015721-e0015721 (2021-06-02)
Zebrafish (Danio rerio) is an attractive model organism to use for an array of scientific studies, including host-microbe interactions. Zebrafish contain a core (i.e., consistently detected) intestinal microbiome consisting primarily of Proteobacteria. Furthermore, this core intestinal microbiome is plastic and
Paul Breen et al.
Infection and immunity, 89(9), e0015121-e0015121 (2021-06-08)
Zebrafish (Danio rerio) are an attractive model organism for a variety of scientific studies, including host-microbe interactions. The organism is particularly useful for the study of aquatic microbes that can colonize vertebrate hosts, including Vibrio cholerae, an intestinal pathogen. V.
Yixiang Zhong et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3), e21379-e21379 (2021-02-11)
Traditionally cultured monolayers of primary human hepatocytes (PHHs) deteriorate within days and thereby become unsuitable for drug-related studies. PHH spheroids (3D PHHs) maintain liver functions for weeks, but are considerably more demanding. Recently, a chemical-based approach (5C PHHs) succeeded in

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