2,5-Dihydroxybenzoic acid

matrix substance for MALDI-MS, >99.0% (HPLC)

Gentisic acid, 2,5-DHBA, DHB, Hydroquinonecarboxylic acid
Linear Formula:
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


matrix substance for MALDI-MS


>99.0% (HPLC)

analyte functional class(es)


analyte chemical class(es)

glycans, lipids, organic molecules, peptides, proteins


MALDI-MS: suitable


200-207 °C
204-208 °C (lit.)

cation traces

Ba: ≤5 mg/kg
Ca: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
K: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

ε (extinction coefficient)

>2000 at 337 nm

SMILES string




InChI key


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2,5-Dihydroxybenzoic acid is an ionic liquid matrix used for the analysis of biomolecules using matrix-assisted laser desorption/ionization combined with mass spectrometry.
Most commonly used matrix for carbohydrates. For MALDI-MS of free neutral glycans. Produces [M+Na]+ ion; may show a weaker [M+K]+ ion. Other species may be generated by the addition of the appropriate inorganic salt.


11 - Combustible Solids

WGK Germany


Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

2,5-Dihydroxybenzoic acid butylamine and other ionic liquid matrixes for enhanced MALDI-MS analysis of biomolecules
Mank M, et al.
Analytical Chemistry, 76(10), 2938-2950 (2004)
Matrix-assisted laser desorption/ionization mass spectrometry with additives to 2,5-di-hydroxy-benzoic acid.
Karas, M., et al.,
Org. Mass Spectrom., 28, 1476-1481 (1993)
Naoki Sato
Plant & cell physiology, 56(10), 1890-1899 (2015-08-16)
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B E Cham et al.
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We have developed a specific and sensitive method for the determination of salicylic acid, salicyluric acid, and gentisic acid in urine. Any proteins present are precipitated with methyl cyanide. After centrifugation, an aliquot of the supernate is directly injected into...
Colin Correnti et al.
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