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85878

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

form

powder

Quality Level

quality

affinity purified
lyophilized from 10 mM potassium phosphate

specific activity

≥13 U/mg protein

mol wt

Mr ~60000

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

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General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Application

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

Unit Definition

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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