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86186

Sigma-Aldrich

Sulforhodamine B acid chloride

suitable for fluorescence, technical

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Synonym(s):
Lissamine rhodamine B sulfonylchloride
Empirical Formula (Hill Notation):
C27H29ClN2O6S2
CAS Number:
Molecular Weight:
577.11
Beilstein:
7734927
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

technical

Quality Level

form

powder

fluorescence

λex 560 nm; λem 580 nm in chloroform
λex 560 nm; λem ~580 nm in chloroform

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCN(CC)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(Cl)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3

InChI key

YERWMQJEYUIJBO-UHFFFAOYSA-N

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This Item
S901234173883689
Rhodamine B for fluorescence

Sigma-Aldrich

83689

Rhodamine B

form

powder

form

powder

form

solid

form

powder or crystals

fluorescence

λex 560 nm; λem 580 nm in chloroform, λex 560 nm; λem ~580 nm in chloroform

fluorescence

-

fluorescence

-

fluorescence

λex 553 nm; λem 627 nm in methanol

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

room temp

storage temp.

-

storage temp.

-

General description

Sulforhodamine B is a fluorescent dye.

Application

Sulforhodamine B acid chloride has been used to synthesize rhodamine-labeled polycation (PLL-ROD). It has also been used in fluorescent protein tracer preparation.
Fluorescent protein label forming stable conjugates

Biochem/physiol Actions

The sulforhodamine B (SRB) assay is useful in determining the density of cells.

Legal Information

Lissamine is a trademark of PerkinElmer Inc.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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