86826

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

• Synonym(s): 5-FAM
• Empirical Formula (Hill Notation): C₂₁H₁₂O₇
• CAS Number: 76823-03-5
• Molecular Weight: 376.32
• Beilstein: 57037
• MDL number: MFCD00036874
• PubChem Substance ID: 57653195

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: powder
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• SMILES string: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves