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86826

Sigma-Aldrich

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

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Synonym(s):
5-FAM
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
Beilstein/REAXYS Number:
57037
MDL number:
PubChem Substance ID:

product line

BioReagent

Quality Level

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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5-Carboxyfluorescein BioReagent, suitable for fluorescence, ≥95% (HPLC)

86826

5-Carboxyfluorescein

6-Carboxyfluorescein ~97% (HPLC)

C0662

6-Carboxyfluorescein

5(6)-Carboxyfluorescein BioReagent, suitable for fluorescence, ≥95% (HPLC)

21877

5(6)-Carboxyfluorescein

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

fluorescence

λex 492 nm; λem 517 nm (pH 8.0)

fluorescence

λex 492 nm; λem 517 nm in DMF

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

solubility

DMF: soluble, H2O: soluble, DMSO: soluble

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

solubility

DMF: soluble, DMSO: soluble

solubility

-

form

powder

form

powder

form

powder

form

powder

assay

≥95% (HPLC)

assay

~97% (HPLC)

assay

≥80% (HPLC)

assay

≥95% (HPLC)

product line

BioReagent

product line

-

product line

BioReagent

product line

BioReagent

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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The loss-of-allele assay for ES cell screening and mouse genotyping
Methods in Enzymology, 476, 295-307 (2010)
Intracellular pH and its Measurement (1989)
Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes
Hudiyanti D, et al.
Orient. J. Chem., 31, 435-439 (2015)
Detection of Bacteria, Viruses, Parasites and Fungi (2010)
Peilin Yang et al.
Analytical chemistry, 77(8), 2482-2489 (2005-04-15)
Fluorescence anisotropy capillary electrophoresis (FACE) and affinity probe capillary electrophoresis (APCE) with laser-induced fluorescence detection were evaluated for analysis of peptide-protein interactions with rapid binding kinetics. The Src homology 2 domain of protein SH2-Bbeta (SH2-Bbeta (525-670)) and a tyrosine-phosphorylated peptide

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