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86826

Sigma-Aldrich

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):
5-FAM
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
Beilstein:
57037
MDL number:
PubChem Substance ID:

Quality Level

product line

BioReagent

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

Intracellular pH and its Measurement (1989)
The loss-of-allele assay for ES cell screening and mouse genotyping
Methods in Enzymology, 476, 295-307 (2010)
Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes
Hudiyanti D, et al.
Orient. J. Chem., 31, 435-439 (2015)
Detection of Bacteria, Viruses, Parasites and Fungi (2010)
Christian Dittrich et al.
Macromolecular bioscience, 10(12), 1406-1415 (2010-12-18)
CD3ac, an uncharged and strongly hydrophobic 10 amino acid peptide (Ac-LK(Ac)-LK(Ac)-LK(Ac)-LW-DL-LW-DL-LW-DL-LW-NH2) was synthesized and purified. The peptide readily dissolves in ethanol and--upon solvent exchange to water--assembles into solid spherical particles with diameters of around 500 nm and low size-polydispersity. CD3ac

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