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87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

suitable for fluorescence, ≥95.0% (HPLC)

Synonym(s):

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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Size/SKUAvailabilityPrice
5 mg

Available to ship TODAYfromMILWAUKEE

$237.00

About This Item

Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
150206-05-6
Molecular Weight:
557.46
NACRES:
NA.32
PubChem Substance ID:
57653248
UNSPSC Code:
12352108
MDL number:
MFCD00037466

$237.00

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Quality Segment

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy

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This Item
H17593680155156
5-Carboxy-fluorescein diacetate N-succinimidyl ester suitable for fluorescence, ≥95.0% (HPLC)

Sigma-Aldrich

87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

(+)-Biotin N-hydroxysuccinimide ester ≥98% (HPLC), powder

Sigma-Aldrich

H1759

(+)-Biotin N-hydroxysuccinimide ester

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

Sigma-Aldrich

36801

7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)

Sigma-Aldrich

55156

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester

description

suitable for fluorescence, ≥95.0% (HPLC)

description

≥98% (HPLC), powder

description

BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

description

suitable for fluorescence, ≥95.0% (coupling assay to aminopropyl silica gel)

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence, corresponds for coupling to amines

suitability

suitable for fluorescence

fluorescence

λex 492 nm; λem 517 nm

fluorescence

-

fluorescence

λex 445 nm; λem 482 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 386 nm; λem 448 nm in 0.1 M Tris pH 9.0

form

powder

form

powder

form

-

form

-

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble

solubility

DMF: soluble, acetonitrile: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥95.0% (HPLC)

assay

≥98% (HPLC)

assay

≥96.0% (HPLC)

assay

≥95.0% (coupling assay to aminopropyl silica gel)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–3 of 3 Questions  

  1. Can user the product 5-Carboxy-fluorescein diacetate N-succinimidyl ester 87444 in cells living infected with parasite?

    1 answer

    1. This application will need to be validated by the end user. This product is passively taken up by cells. Fluorescence occurs when the dye is hydrolyzed by esterases within the cell. The dye reacts covalently with amine groups present on intercellular proteins, which allows for long-term staining effects. This dye may be taken up by the parasite, as well.

      The link below may offer additional helpful information:
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/carboxyfluorescein-diacetate-succinimidyl-ester

      Helpful?

  2. Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?

    1 answer

    1. Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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