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87444

Sigma-Aldrich

5-Carboxy-fluorescein diacetate N-succinimidyl ester

suitable for fluorescence, ≥95.0% (HPLC)

Synonym(s):

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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5 MG
$220.00

About This Item

Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
150206-05-6
Molecular Weight:
557.46
MDL number:
MFCD00037466
UNSPSC Code:
12352108
PubChem Substance ID:
57653248
NACRES:
NA.32

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$220.00

In StockDetails

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assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

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5-Carboxy-fluorescein diacetate N-succinimidyl ester suitable for fluorescence, ≥95.0% (HPLC)

87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

6-Carboxyfluorescein diacetate ≥95% (HPLC)

C5041

6-Carboxyfluorescein diacetate

5(6)-Carboxyfluorescein diacetate BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

21879

5(6)-Carboxyfluorescein diacetate

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)

46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

grade

for fluorescence

grade

-

grade

-

grade

-

fluorescence

λex 492 nm; λem 517 nm

fluorescence

-

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 490 nm; λem 514 nm (pH 8.2), λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

form

powder

form

powder

form

powder

form

solid

solubility

DMF: soluble, DMSO: soluble

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

DMSO: soluble

solubility

DMF: soluble, methanol: soluble

assay

≥95.0% (HPLC)

assay

≥95% (HPLC)

assay

≥90.0% (HPLC)

assay

≥75% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry[1]; labeling human intervertebral disk cells in vitro for fluorescence microscopy[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL9002
BCCH7934
BCCJ7672
BCCD0002
BCCB9167

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Questions

1–3 of 3 Questions  

  1. Can user the product 5-Carboxy-fluorescein diacetate N-succinimidyl ester 87444 in cells living infected with parasite?

    1 answer

    1. This application will need to be validated by the end user. This product is passively taken up by cells. Fluorescence occurs when the dye is hydrolyzed by esterases within the cell. The dye reacts covalently with amine groups present on intercellular proteins, which allows for long-term staining effects. This dye may be taken up by the parasite, as well.

      The link below may offer additional helpful information:
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/carboxyfluorescein-diacetate-succinimidyl-ester

      Helpful?

  2. Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?

    1 answer

    1. Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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