Tetramethylrhodamine ethyl ester perchlorate

Name: Tetramethylrhodamine ethyl ester perchlorate
Brand: SIGMA
Price: 226 USD

suitable for fluorescence, ≥90% (HPCE)

Synonym(s):

TMRE, TMRE perchlorate

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25 MG

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About This Item

Empirical Formula (Hill Notation):

C₂₆H₂₇ClN₂O₇

CAS Number:

115532-52-0

Molecular Weight:

514.95

MDL number:

MFCD00467973

UNSPSC Code:

12352116

PubChem Substance ID:

329769616

NACRES:

NA.32

Key Documents

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assay

≥90% (HPCE)

form

solid

solubility

DMSO: soluble
alcohols: soluble

fluorescence

λ_ex 540 nm; λ_em 595 nm in DMSO

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.CCOC(=O)c1ccccc1-c2c3ccc(cc3[o+]c4cc(ccc24)N(C)C)N(C)C

InChI

1S/C26H27N2O3.ClHO4/c1-6-30-26(29)20-10-8-7-9-19(20)25-21-13-11-17(27(2)3)15-23(21)31-24-16-18(28(4)5)12-14-22(24)25;2-1(3,4)5/h7-16H,6H2,1-5H3;(H,2,3,4,5)/q+1;/p-1

InChI key

NBAOBNBFGNQAEJ-UHFFFAOYSA-M

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Show Differences

1 of 4

This Item	T5428	E-006	546062
Sigma-Aldrich 87917 Tetramethylrhodamine ethyl ester perchlorate	Sigma-Aldrich T5428 Tetramethylrhodamine methyl ester perchlorate	Supelco E-006 EDDP perchlorate	Sigma-Aldrich 546062 Rhodamine 19 perchlorate
suitability suitable for fluorescence	suitability -	suitability -	suitability -
fluorescence λ_ex 540 nm; λ_em 595 nm in DMSO	fluorescence λ_em 573 nm in methanol	fluorescence -	fluorescence λ_em 544 nm in ethanol
solubility DMSO: soluble, alcohols: soluble	solubility methanol: 5 mg/mL, clear	solubility -	solubility -
form solid	form powder	form solid	form crystalline
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level 100
assay ≥90% (HPCE)	assay ≥95%	assay -	assay -

General description

Tetramethylrhodamine ethyl ester perchlorate (TMRE), also known as TMRE perchlorate, is a potential mitochondrial dye. TMRE staining process is reversible, and it doesn’t affect cell proliferation and viability.[1]

The sorted cells containing TMRE perchlorate don’t interfere with the subsequent functional assays and hence are a preferred choice for the enrichment of functionally active, unbiased cell populations.[1]

Application

Tetramethylrhodamine ethyl ester perchlorate (TMRE) is used to study the effects of high glucose in inducing mitochondrial morphology and metabolic changes.[2]

TMRE perchlorate is also used to study Fullerene C 60 penetration into Leukemic Cells and the subsequent photoinduced Cytotoxic Effects.[3]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Potential-sensitive probe for measuring membrane potential changes in mitochondria[4]

pictograms

GHS07

signalword

Warning

hcodes

H302 + H332,H315,H319

pcodes

P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Downregulation of sirtuin 3 by palmitic acid increases the oxidative stress, impairment of mitochondrial function, and apoptosis in liver cells.

Garima Sharma et al.

Journal of biochemical and molecular toxicology, 33(8), e22337-e22337 (2019-04-09)

Elevated levels of saturated fatty acids show a strong cytotoxic effect in liver cells. Sirtuin 3 (SIRT3), a mitochondrially localized member of NAD+ -dependent deacetylase has been shown to protect hepatocytes against the oxidative stress. The role of SIRT3 on

p53-Bad: A Novel Tumor Suppressor/Proapoptotic Factor Hybrid Directed to the Mitochondria for Ovarian Cancer Gene Therapy.

Phong Lu et al.

Molecular pharmaceutics, 16(8), 3386-3398 (2019-06-27)

Clinical trials involving p53 gene therapy for ovarian cancer failed due to the dominant negative inhibition of wild-type p53 and multiple genetic aberrations in ovarian cancer. To overcome this problem, we have designed a more potent chimeric gene fusion, called

High glucose induces mitochondrial morphology and metabolic changes in retinal pericytes.

Kyle Trudeau et al.

Investigative ophthalmology & visual science, 52(12), 8657-8664 (2011-10-08)

Mitochondrial dysfunction is known to play a role in retinal vascular cell loss, a prominent lesion of diabetic retinopathy. High glucose (HG) has been reported to induce mitochondrial fragmentation and dysfunction in retinal endothelial cells, contributing to apoptosis. In this

EDNRB isoform 3 confers Temozolomide resistance in A375 melanoma cells by modulating membrane potential, reactive oxygen species and mitochondrial Ca2.

Yun Shan Chen et al.

Cancer management and research, 11, 7353-7367 (2019-09-10)

The role of endothelin receptor type B (EDNRB) isoform 3 involved in Temozolomide (TMZ)-induced melanoma cell death has not yet been elucidated. The subcellular localization of EDNRB isoform 3 was determined by confocal and immunoblotting assays. Silencing EDNRB isoform 3

Mitochondrial staining allows robust elimination of apoptotic and damaged cells during cell sorting.

Natasha S Barteneva et al.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 62(4), 265-275 (2014-01-08)

High-speed fluorescence-activated cell sorting is relevant for a plethora of applications, such as PCR-based techniques, microarrays, cloning, and propagation of selected cell populations. We suggest a simple cell-sorting technique to eliminate early and late apoptotic and necrotic cells, with good

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