Tetramethylrhodamine isothiocyanate, also known as TRITC is a fluorescent antibody labelling agent. It is a red-fluorescing marker and can be tracked through fluorescence microscopy. TRITC dissolves completely in DMSO at a rapid rate. And gamma globulin is unaffected to up to 30% DMSO dilution in carbonate buffer. Tetramethylrhodamine isothiocyanate doesn’t dissolve in other denaturing agents completely.
Tetramethylrhodamine isothiocyanate or TRITC is used for tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection. It is an extremely intensive fluorochrome used for labeling antibodies.
An extremely intensive fluorochrome for labeling antibodies