88021102

Chang Liver (HeLa derivative) cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human cervix, Epithelial

• Synonym(s): Chang Liver cell line (HeLa derivative)
• UNSPSC Code: 41106514
• Biological source: human cervix
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal no. 70
• Morphology: Epithelial

Properties

• biological source: human cervix
• Quality Segment: 100
• form: liquid
• packaging: tube of 5 μg 88021102-DNA-5UG, pkg of vial of cells 88021102-1VL
• growth mode: Adherent
• karyotype: Modal no. 70
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer

Description

Application

Virus, biochemical and replication studies

Chang Liver (HeLa derivative) cell line has been used to study the construction and biological activity of human torque teno virus (TTV) genotype 6.

Biochem/physiol Actions

Human cervix carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,14
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable