90819

Sigma-Aldrich

Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

Synonym(s):
Ru(bpy)2(phen-5-NH2)(PF6)2
Empirical Formula (Hill Notation):
C32H25N7Ru · 2 PF6
CAS Number:
Molecular Weight:
898.59
MDL number:
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.

product line

BioReagent

form

powder

reaction suitability

core: ruthenium
reaction type: Photocatalysis
reagent type: catalyst

application(s)

hematology: suitable
histology: suitable

fluorescence

λex 458 nm; λem 612 nm in 0.1 M Tris pH 7.0

suitability

suitable for fluorescence

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

−20°C

SMILES string

Ru++.FP-(F)(F)(F)(F)F.FP-(F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Nc5cc6cccnc6c7ncccc57

InChI

1S/C12H9N3.2C10H8N2.2F6P.Ru/c13-10-7-8-3-1-5-14-11(8)12-9(10)4-2-6-15-12;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h1-7H,13H2;2*1-8H;;;/q;;;2*-1;+2

InChI key

QQMMCXKCYXQNNA-UHFFFAOYSA-N

Application

Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.

Features and Benefits

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.
These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes′ shifts.
In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Photoinduced intramolecular electron transfer in peptide-bridged molecules

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
E Terpetschnig et al.
Analytical biochemistry, 251(2), 241-245 (1997-09-23)
We report the synthesis and spectral properties of a long-lifetime luminescent Ru complex containing a sulfhydryl-reactive maleimide group, [Ru (2,2'-bipyridine)2(1, 10-phenanthroline-5-maleimide)](PF6)2. [Ru(bpy)2(phen-mi)]2+ was covalently linked to human serum albumin, immunoglobulin G, and beta-galactosidase. The lifetimes for probe bound to proteins...
Tao Wang et al.
Analytica chimica acta, 1062, 124-130 (2019-04-06)
Vibrio parahaemolyticus (VP), as a kind of harmful bacterium to human beings, its rapid and accurate detection is particularly important. Herein, we developed a simple and efficient dual-modal Faraday cage-type immunosensor for ultrasensitive detection of VP based on electrochemiluminescence (ECL)...
K.S. Schanze et al.
Journal of the American Chemical Society, 110, 1180-1180 (1988)

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