90909C

HEPES

dry powder

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 329769965

Properties

• description: for research or for further manufacturing use
• form: dry powder
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Application

HEPES is added in many cell culture media. It was used to prepare cell suspension for flow cytometry analysis of cardiac progenitor cells.

HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37°C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

Packaging

1 pkg

25, 100, 500 g

1, 5 kg

Biochem/physiol Actions

HEPES has buffering capacity in the pH range of 7.2 - 7.4. It is added frequently to cell culture media in addition to sodium bicarbonate at 10 mM to 25 mM concentration.