91228

Sigma-Aldrich

Trichloroacetic acid

BioUltra, ≥99.5% (T)

Synonym(s):
TCA
Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
Beilstein/REAXYS Number:
970119
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

vapor density

<1 (vs air)

vapor pressure

1 mmHg ( 51 °C)

product line

BioUltra

assay

≥99.5% (T)

form

crystals
flakes

impurities

insoluble matter, passes filter test

ign. residue

≤0.05%

refractive index

n20/D 1.62 (lit.)

pH

<1.0 (25 °C, 0.5 M in H2O)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤30 mg/kg
nitrate (NO3-): ≤20 mg/kg
phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

SMILES string

OC(=O)C(Cl)(Cl)Cl

UV absorption

λ: 260 nm Amax: 1.60
λ: 280 nm Amax: 0.15

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

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General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Application

Trichloroacetic acid (TCA) has been used:
  • to determine its effect on avian embryos during an exposure window encompassing cardiac specification
  • as a cell fixative
  • to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
  • for the precipitation of acidic ribosomal proteins (RPs)

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Signal Word

Danger

Hazard Statements

hazcat

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

storage_class_code

8B - Non-combustible, corrosive hazardous materials

WGK Germany

WGK 2

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Cardiogenic effects of trichloroethylene and trichloroacetic acid following exposure during heart specification of avian development
Drake V, et al.
Toxicological Sciences, 94(1), 153-162 (2006)
Efficacy of 5-FU or oxaliplatin monotherapy over combination therapy in colorectal cancer
Toloudi M, et al.
Journal of Cancer Therapy, 6(04), 345-345 (2015)
CHCHD4 confers metabolic vulnerabilities to tumour cells through its control of the mitochondrial respiratory chain
Thomas L, et al.
Cancer & Metabolism, 7(1), 2-2 (2019)
Sulforhodamine B (SRB) assay in cell culture to investigate cell proliferation
Orellana E and Kasinski A
Bio-protocol, 6(21) (2016)
M.K. Dutt
Science and Culture, 51, 196-196 (1985)

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