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MilliporeSigma

93289

Sigma-Aldrich

TRI Reagent®

Synonym(s):

DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent

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25 ML
$116.00
100 ML
$353.00

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25 ML
$116.00
100 ML
$353.00

About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.52

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$116.00


In StockDetails


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form

liquid

Quality Level

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue

impurities

phenol

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This Item
T9424T3934TRIPURE-RO
grade

for DNA, RNA and protein isolation

grade

-

grade

-

grade

-

form

liquid

form

-

form

-

form

solution (ready-to-use)

usage

 mL sufficient for 107 cells

usage

 mL sufficient for 107 cells,  mL sufficient for 100 mg tissue (or)

usage

0.75 mL sufficient for 0.25 mL fluid samples

usage

-

impurities

phenol

impurities

-

impurities

-

impurities

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA.[1] This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.

Application

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
TRI Reagent® has been used for isolating RNA from various types of cells or tissues.[2][3][4] It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

target_organs

Nervous system,Kidney,Liver,Skin

supp_hazards

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 2

flash_point_f

174.2 °F - closed cup

flash_point_c

79 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Questions

  1. Excuse me, what is the principle of trizol reagent to separate DNA and RNA?

    1 answer
    1. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. Please see the link below to review further information as well as review specific protocols:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/543/t9424bulletin.pdf

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