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93289

Sigma-Aldrich

TRI Reagent®

for DNA, RNA and protein isolation

Synonym(s):
DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent
MDL number:
NACRES:
NA.52

grade

for DNA, RNA and protein isolation

Quality Level

form

liquid

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue

impurities

phenol

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93289

TRI Reagent®

TRI Reagent® For processing tissues, cells cultured in monolayer or cell pellets

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T9424

TRI Reagent®

TRI Reagent® BD, For processing whole blood, plasma, or serum.

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T3809

TRI Reagent®

Sigma-Aldrich

Sigma-Aldrich

PNS1010

Stabilyser Reagent

grade

for DNA, RNA and protein isolation

grade

-

grade

-

grade

-

form

liquid

form

-

form

-

form

liquid

usage

 mL sufficient for 107 cells

usage

 mL sufficient for 107 cells,  mL sufficient for 100 mg tissue (or)

usage

0.75 mL sufficient for 0.25 mL blood derivatives

usage

-

impurities

phenol

impurities

-

impurities

-

impurities

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.

Application

TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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