This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. Please see the link below to review further information as well as review specific protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/543/t9424bulletin.pdf
93289
TRI Reagent®
Synonym(s):
DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent
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$116.00
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$116.00
In StockDetails
form
liquid
Quality Level
usage
mL sufficient for 107 cells
mL sufficient for 100 mg tissue
impurities
phenol
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This Item | T9424 | T3934 | TRIPURE-RO |
---|---|---|---|
grade for DNA, RNA and protein isolation | grade - | grade - | grade - |
form liquid | form - | form - | form solution (ready-to-use) |
usage mL sufficient for 107 cells | usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or) | usage 0.75 mL sufficient for 0.25 mL fluid samples | usage - |
impurities phenol | impurities - | impurities - | impurities - |
Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
General description
Application
Features and Benefits
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
Legal Information
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk_germany
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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