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93337

Sigma-Aldrich

Trizma® acetate

BioUltra, ≥99.0% (NT)

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Synonym(s):
TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt
Linear Formula:
NH2C(CH2OH)3 · CH3COOH
CAS Number:
Molecular Weight:
181.19
Beilstein/REAXYS Number:
3702918
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

assay

≥99.0% (NT)

form

crystalline powder

impurities

insoluble matter, passes filter test

ign. residue

≤0.2%

loss

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

SMILES string

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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This Item
T12589332893348
vibrant-m

93337

Trizma® acetate

Premium Grade
vibrant-m

T1258

Trizma® acetate

-
vibrant-m

93328

Trizma® maleate

Premium Grade
vibrant-m

93348

Trizma® phosphate monobasic

Premium Grade
assay

≥99.0% (NT)

assay

≥99.0% (titration)

assay

≥99.5% (NT)

assay

≥99.0% (NT)

product line

BioUltra

product line

-

product line

BioUltra

product line

BioXtra

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

anion traces

-

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

water: 0.5 M, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear

impurities

insoluble matter, passes filter test

impurities

-

impurities

insoluble matter, passes filter test

impurities

-

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Other Notes

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
Patrick S Aranda et al.
Electrophoresis, 33(2), 366-369 (2012-01-10)
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Chi-Lin Li et al.
The Analyst, 137(22), 5222-5228 (2012-10-04)
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation

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