Phosphate Buffer Saline (PBS) and Tris Buffer Saline (TBS) are concentrated wash solutions designed for rinsing reagents from tissue slides and preserving tissue sections between reagent applications. PBS is recommended with any detection system, except those containing alkaline phosphatase. Conversely, TBS is recommended when alkaline phosphatase is present to avoid excessive phosphate accumulation and consequent reduction in the rate of reaction. Adding Tween® 20 to both PBS and TBS may help reduce background and produce a cleaner immunohistochemical stain overall. The most significant factor is the intention for use to visualize the stain, with what chromogen and detection kit. For HRP or Alk Phos on the breast panel, TBS can be used for both. For diluent, as long as products are stored properly (2 to 8C), the diluent will help stabilize diluted antibodies, and the same applies for use with HRP or Alk Phos detection for TBS to be run on either, while PBS works best with HRP. This allows for a longer expiration date and aligns with the antibody expiration date.
935B
TBS IHC Wash Buffer + TWEEN® 20
20X TBS, 1% Tween® 20
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This Item | |||
|---|---|---|---|
| technique(s) immunohistochemistry: suitable | technique(s) - | technique(s) - | technique(s) cell culture | plant: suitable, microbiological culture: suitable |
| Quality Level 500, 600 | Quality Level 100 | Quality Level 200 | Quality Level - |
| form buffered aqueous solution | form tablet | form solution | form solution |
| storage temp. 2-8°C | storage temp. 15-25°C | storage temp. room temp | storage temp. 2-8°C |
| description [For In Vitro Diagnostic Use in Select Regions] | description - | description Saline-Sodium Citrate buffer, made with ultrapure water | description Formula: C58H114O26, for n = 20 |
| packaging pkg of 1 L 20x (935B-09) | packaging - | packaging - | packaging pkg of 5 × 10 mL |
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Biochem/physiol Actions
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12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is there a recommended or preferable wash buffer (TBS or PBS) for a breast panel in Autostainer? Moreover, please confirm how long one can wait once the dilution of the concentrated product is done? Both TBS and PBS in this case.
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