viscosity 250-450 mPa.s (25 °C)

Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
MDL number:

Quality Level




viscous liquid

mol wt



DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

refractive index

n20/D 1.468 (lit.)
n20/D 1.471


250-450 mPa.s(25 °C)

HLB value



1.11 g/mL at 20 °C
1.095 g/mL at 25 °C (lit.)



InChI key


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General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.


TWEEN 20 is widely used in several biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
  • for lysing mammalian cells at a concentration of 0.005 to 0.5%
  • For rehydrating tissue before in situ hybridization
Non-ionic detergent


may get turbid on storage, heat to clear

Legal Information

TWEEN is a registered trademark of Croda International PLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

527.0 °F - Pensky-Martens closed cup

Flash Point(C)

275 °C - Pensky-Martens closed cup

Certificate of Analysis

Certificate of Origin

Reynolds JEF
Martindale: The Extra Pharmacopoeia, 1030-1030 (1993)
Enhanced p62 expression triggers concomitant autophagy and apoptosis in a rat chronic spinal cord compression model.
Chen Z, et al.
Molecular Medicine Reports, 9(6), 2091-2096 (2014)
The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis.
Snapkov I, et al.
BMC Cancer, 16(1), 490-490 (2016)
Temporal expression of cytokines and signal transducer and activator of transcription factor 3 activation after neonatal hypoxia/ischemia in mice.
Snapkov I, et al.
Developmental Neuroscience, 35(2-3), 212-225 (2013)
Procedures for whole-mount immunohistochemistry and in situ hybridization of immature mammalian CNS.
Luque JM, et al.
Brain Res. Protoc., 2(2), 165-173 (1998)

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