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94149

Sigma-Aldrich

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):
Streptavidin-Atto 647N
NACRES:
NA.25

product line

BioReagent

Quality Level

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 650.0-656.0 nm Amax

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

General description

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Application

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Features and Benefits

Atto 647N- Streptavidin has the following benefits-

  • Highly photostable. Strong absorption.
  • Excellent fluorescent quantum yield.
  • Highly resistant to ozone degradation.
  • Minimal triplet formation.

Analysis Note

dye-to-protein ratio ≥1.5

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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