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94149

Sigma-Aldrich

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

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Synonym(s):
Streptavidin-Atto 647N
NACRES:
NA.25

product line

BioReagent

Quality Level

100

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 650.0-656.0 nm Amax

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

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Atto 647N-Streptavidin

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form

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form

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form

solid

suitability

corresponds for gel electrophoresis, suitable for fluorescence

suitability

corresponds for gel electrophoresis, suitable for fluorescence

suitability

-

suitability

corresponds for gel electrophoresis, suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

λ

-

λ

-

λ

in PBS (buffer pH 7.4)

General description

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Application

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Features and Benefits

Atto 647N- Streptavidin has the following benefits-

  • Highly photostable. Strong absorption.
  • Excellent fluorescent quantum yield.
  • Highly resistant to ozone degradation.
  • Minimal triplet formation.

Analysis Note

dye-to-protein ratio ≥1.5

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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