94742

Vanadyl ribonucleoside complexes solution

BioReagent, Molecular Biology, 200 mM

• Synonym(s): Ribonucleoside vanadyl complexes
• UNSPSC Code: 12352200
• NACRES: NA.52

Properties

• grade: Molecular Biology
• Quality Level: 100
• product line: BioReagent
• form: liquid
• concentration: 200 mM
• impurities: oxovanadium V
• suitability: in accordance for ribonuclease inhibition test
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]

Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase).[2] Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.[3]

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.

Vanadyl ribonucleoside complexes solution has been used:

• as a component of polysome lysis buffer[4] and fluorescence in situ hybridization (FISH) buffer[5][6]
• as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes[7]

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable