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94875

Sigma-Aldrich

Atto 680

BioReagent, suitable for fluorescence, ≥90% (HPLC)

MDL number:
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

InChI

1S/C27H31N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-15H,4-10,16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

MHHMNDJIDRZZNT-UHFFFAOYSA-N

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94875

Atto 680

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Atto 700 NHS-ester

assay

≥90% (HPLC)

assay

≥85% (HPLC)

assay

≥80% (sum of isomers, HPCE)

assay

≥90% (coupling to amine, HPLC)

form

powder

form

powder

form

solid

form

powder

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide

fluorescence

λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Find more information here

Application

Atto 680 has been used as a dye to study the fluorescence quantum yields of bioanalytically relevant red dyes.
Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 680 shows a molar extinction of 120,000 and QY of 30% in water (40% in ethanol). Decay time is 1.8 ns.

Other Notes

New red absorbing fluorescent dye as a biophysical probe for binding interactions; used as fluorescence tracer in spray diagnostics.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared
Wu?rth C, et al.
Analytical Chemistry, 84(3), 1345-1352 (2012)
Dual laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions - evaluation of new red fluorescent dyes.
K.M. Swift et al.
Proc. SPIE: Int. Soc. Opt. Eng., 4252, 47-47 (2001)
Spray diagnostics using an all-solid-state Nd:YAIO3 laser and fluorescence tracers in commercial gasoline and diesel fuels.
I. Duwell et al.
Applied Physics B: Lasers and Optics, 79, 249-254 (2004)
Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650
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Biochemical and biophysical research communications, 363(1), 24-29 (2007-09-11)
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Fluorescence Lifetime Measurement

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