Vitamin B12a hydrochloride

≥96% (UV), ≥96% (HPLC)

Hydroxocobalamin hydrochloride
Empirical Formula (Hill Notation):
C62H89CoN13O15P · HCl
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source



≥96% (HPLC)
≥96% (UV)


powder or crystals


≤12% loss on drying, 20 °C (HV)


red to dark red


methanol: 10 mg/mL at 20 °C, clear, dark red

storage temp.


SMILES string




InChI key


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Vitamin B12a hydrochloride has been used as an antidote for cyanide poisoning.

Biochem/physiol Actions

Vitamin B12 (cobalamin) refers to a group of chemically-related cobalt containing molecules involved in cell processes such as DNA synthesis, fatty acid synthesis, energy production and regulation. The physiologically active forms of vitamin B12 include methylcobalamin and adenosylcobalamin, whereas hydroxocobalamin (vitamin B12a, OHCbl) and cyanocobalamin (CNCbl) are storage and delivery forms. Bacteria-derived hydroxocobalamin (OHCbl) and CNCbl are converted in humans to useful coenzyme forms to support metabolic processes such as mitochondrial methylmalonylcoenzyme A mutase conversion of methylmalonic acid (MMA) to succinate to link lipid and carbohydrate metabolism, and activation of methionine synthase, the rate limiting step in the synthesis of methionine.

Other Notes


Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Cisplatin analogs confer protection against cyanide poisoning
Nath AK, et al.
Cell Chemical Biology, 24(5), 565-575 (2017)
S. Fukui et al.
Antibiotics, Vitamins and Hormones, 101-101 (1977)
R Pace et al.
Clinical toxicology (Philadelphia, Pa.), 52(7), 647-650 (2014-08-05)
Pre-hospital administration of hydroxocobalamin (B12a) is used for empiric treatment of cyanide poisoning because cyanide poisoning is difficult to identify and requires immediate treatment. B12a interferes with the accuracy of several blood laboratory tests. This study aimed to explore how...
Mamoru Yamanishi et al.
Trends in biochemical sciences, 30(6), 304-308 (2005-06-14)
Many organic cofactors are both rare and reactive. They are usually in low abundance, which poses problems for efficient collision-based targeting to dependent enzymes, whereas their reactivity is problematic for side reactions. Sequestration and escorted delivery presents one solution to...
Jasmin Federizon et al.
Pharmaceutics, 13(1) (2021-01-21)
Cobalt porphyrin phospholipid (CoPoP) can incorporate within bilayers to enable non-covalent surface-display of antigens on liposomes by mixing with proteins bearing a polyhistidine tag (his-tag); however, the mechanisms for how this occurs are poorly understood. These were investigated using the...

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