it is one drop of chromogen to 1ml of substrate buffer.
957D
DAB Substrate Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.32
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description
For In Vitro Diagnostic Use in Select Regions
Quality Level
form
aqueous solution
packaging
pkg of 1000 tests (957D-60)
pkg of 150 tests (957D-50)
pkg of 2000 tests (957D-30)
pkg of 500 tests (957D-20)
pkg of 5000 tests (957D-40)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry: suitable
storage temp.
2-8°C
1 of 4
This Item | 954D | 962D | 960D |
|---|---|---|---|
| technique(s) immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable |
| manufacturer/tradename Cell Marque® | manufacturer/tradename Cell Marque® | manufacturer/tradename Cell Marque® | manufacturer/tradename Cell Marque® |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 500 |
| packaging pkg of 1000 tests (957D-60), pkg of 2000 tests (957D-30), pkg of 5000 tests (957D-40), pkg of 150 tests (957D-50), pkg of 500 tests (957D-20) | packaging kit of (7 mL each of predilute amplifier and polymer detector (954D-10)), kit of (containing 100 mL each of predilute amplifier and polymer detector (954D-30)), kit of (containing 1000 mL each of predilute amplifier and polymer detector (954D-40)), kit of (containing 15 mL each of predilute amplifier and polymer detector (954D-50)), kit of (containing 50 mL each of predilute amplifier and polymer detector (954D-20)) | packaging kit of (containing 100 mL each of predilute amplifier and polymer detector (962D-30)), kit of (containing 50 mL each of predilute amplifier and polymer detector (962D-20)), kit of (containing 7 mL each of predilute amplifier and polymer detector (962D-10)) | packaging kit of (containing 0.7 mL each of 3 reagents + 30 mL buffer (960D-10)), kit of (containing 2.3 mL each of 3 reagents + 100 mL buffer (960D-20)) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| description For In Vitro Diagnostic Use in Select Regions | description For In Vitro Diagnostic Use in Select Regions | description For In Vitro Diagnostic Use in Select Regions | description For In Vitro Diagnostic Use in Select Regions |
General description
DAB Substrate Kit is intended for use in immunohistochemistry (IHC) staining protocols. This reagent is designed to detect target antigens in formalin-fixed, paraffin-embedded tissue sections when used in conjunction with primary antibodies and appropriate secondary antibody conjugates in the IHC staining process.When in the presence of HRP polymer, DAB produces a brown precipitate that can be readily visualized using light microscopy. Kits contain: DAB chromogen and DAB buffer substrate.
Quality
United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO
Canada - IVD
European Union - IVD
Japan - RUO
Physical form
Ready to use reagent
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Other Notes
For Technical Service please contact: 800-665-7284 or email: [email protected]
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Muta. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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how to prep DAB?
1 answer-
Helpful?
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Is the absence of color normal when 1 drop of chromagen and 1 ml of substrate are used, given the usual light purple color?
1 answer-
Ensure thorough mixing of the chromagen before combining. The color outcome is influenced by the chromogen's age. A lack of color typically suggests very recent or fresh DAB, which has not undergone oxidation.
Helpful?
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What is the volume for "1 drop" of DAB chromogen?
1 answer-
The volume used to prepare 1ml of DAB would be 0.006mls, rounded to 0.01mls based on calculations for the number of tests in the kit 957D-20.
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