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97875

Sigma-Aldrich

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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MDL number:
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
647 nm

fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 645-651 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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538769534942247
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97875

Atto 647

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53876

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assay

≥90% (HPCE)

assay

-

assay

≥90% (HPLC)

assay

≥80.0% (HPCE), ≥90.0% (HPLC)

form

powder

form

solid

form

solid

form

-

fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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