4-Aza-DL-leucine dihydrochloride

Empirical Formula (Hill Notation):
C5H12N2O2 · 2HCl
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:


≥98.0% (TLC)




white to off-white

storage temp.


SMILES string




InChI key


Biochem/physiol Actions

4-Aza-DL-leucine is used as a leucine analogue that is toxic for bacterial high-affinity branched-chain amino acid (BCAA) transport system (LIV-I). 4-Aza-DL-leucine is used to select mutants of the LIV-1 transporter.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Azusa Saika et al.
AMB Express, 1(1), 6-6 (2011-09-13)
Ralstonia eutropha PHB-4 expressing Pseudomonas sp. 61-3 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1Ps) synthesizes PHA copolymer containing 3-hydroxybutyrate (3HB) and a small amount (0.5 mol%) of 3-hydroxy-4-methylvalerate (3H4MV) from fructose as a carbon source. In this study, enhanced incorporation of 3H4MV...
Feng Wang et al.
Journal of bacteriology, 191(8), 2574-2584 (2009-02-10)
Members of the genus Shewanella inhabit various environments; they are capable of synthesizing various types of low-melting-point fatty acids, including monounsaturated fatty acids (MUFA) and branched-chain fatty acids (BCFA) with and without eicosapentanoic acid (EPA). The genes involved in fatty...
T Hoshino et al.
Journal of bacteriology, 172(10), 5540-5543 (1990-10-01)
About 30 mutants of Pseudomonas aeruginosa PAO defective in the high-affinity branched-chain amino acid transport system (LIV-I) were isolated by the selection for resistance to 4-aza-DL-leucine, a toxic leucine analog for LIV-I. All of the mutants were complemented by plasmid...
Haifeng Su et al.
Scientific reports, 6, 39543-39543 (2016-12-21)
Biosynthesis approaches for the production of higher alcohols as a source of alternative fossil fuels have garnered increasing interest recently. However, there is little information available in the literature about using undirected whole-cell mutagenesis (UWCM) in vivo to improve higher...
Takashi Koyanagi et al.
FEMS microbiology letters, 237(1), 73-77 (2004-07-23)
The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been...

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