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A7512

Sigma-Aldrich

N-Acetyl-DL-phenylalanine β-naphthyl ester

Empirical Formula (Hill Notation):
C21H19NO3
CAS Number:
Molecular Weight:
333.38
MDL number:
PubChem Substance ID:
NACRES:
NA.26

assay

≥98% (TLC)

Quality Level

form

powder

application(s)

ligand binding assay: suitable

color

white

storage temp.

2-8°C

SMILES string

CC(=O)NC(Cc1ccccc1)C(=O)Oc2ccc3ccccc3c2

InChI

1S/C21H19NO3/c1-15(23)22-20(13-16-7-3-2-4-8-16)21(24)25-19-12-11-17-9-5-6-10-18(17)14-19/h2-12,14,20H,13H2,1H3,(H,22,23)

InChI key

BBXRRTJNJCPGBU-UHFFFAOYSA-N

Related Categories

Biochem/physiol Actions

N-Acetyl-DL-phenylalanine β-naphthyl ester (NAPBNE), a chromogenic substrate, is used to identify, differentiate and characterize serine protease(s) and peptidase(s).

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

P Collin-Osdoby et al.
Molecular & general genetics : MGG, 243(6), 674-680 (1994-06-15)
Mutations at the apeA locus in Salmonella typhimurium lead to loss of a soluble enzyme ("protease I") that hydrolyzes the chromogenic endoprotease substrate N-acetyl phenylalanine beta-naphthyl ester. We have isolated pseudorevertants of S. typhimurium apeA mutations that have regained the...
V M Gabert et al.
International journal of pancreatology : official journal of the International Association of Pancreatology, 22(1), 39-43 (1997-08-01)
The results of this study demonstrated that proteolytic enzymes in pancreatic juice from pigs prepared with the pouch method (PM) were nearly fully active or were fully active. When activation with enterokinase was carried out further inactivation and/or breakdown occurred...
K Havemann et al.
Klinische Wochenschrift, 61(1), 49-56 (1983-01-03)
Two cytochemical methods for detection of granulocytic elastase and chymotrypsin employing alanine and phenylalanine naphthyl esters were developed. Specificity of reaction with the ester substrates was proven by chloromethyl ketone inhibitors. The results of both staining methods were almost identical...
H Y Darani et al.
Parasitology, 115 ( Pt 3), 237-247 (1997-09-23)
A cationic Schistosoma mansoni cercarial antigen was shown to be a serine protease as it was capable of hydrolysing N-acetyl-DL-phenylalanine beta-naphthyl ester (NAPBNE) after precipitation by immunoelectrophoresis, and this reaction was modulated by the serine protease inhibitors phenylmethanesulfonyl fluoride (PMSF)...
I Kourteva et al.
Analytical biochemistry, 162(2), 345-349 (1987-05-01)
A technique for quickly detecting nanogram quantities of low- and high-molecular-weight inhibitors of some serine proteases is described. The inhibitor solutions are spotted onto agar films which contain either L-1-p-tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-trypsin or tosyl lysine chloromethyl ketone (TLCK)-chymotrypsin. Enzyme...

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