Documents

A8688

Supelco

Agarase from Pseudomonas atlantica

buffered aqueous glycerol solution

Synonym(s):
Agarose 4-glycanohydrolase, β-Agarase
CAS Number:
Enzyme Commission number:
MDL number:
Pricing and availability is not currently available.

form

buffered aqueous glycerol solution

packaging

vial of 100 units

foreign activity

DNase, RNase and protease, none detected

storage temp.

−20°C

Gene Information

Pseudoalteromonas atlantica T6c ... Patl_1904(4172750), Patl_1971(4172106), Patl_2341(4172495), Patl_2640(4174400), Patl_2642(4174402)

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Application

Retrieves DNA fragments trapped in low-melting point agarose. The recovered DNA fragments are suitable for restriction digest, ligation and cloning.

Biochem/physiol Actions

Agarase digests low melting point agarose by attacking the unsubstituted carbohydrate moieties of neogarobiose agarose (3,6-anhydro-α-L-galactopyranosyl-1,3-D-galactose) and releasing neogaro-oligosaccharides. The mixture of agarase and released carbohydrates does not inhibit restriction endonuclease activity or prevent DNA fragment ligation and transformation.

Suitability

Tested for suitability in isolating λ DNA Hind III fragments from low-melting agarose gels.

Unit Definition

One unit will digest 100 μl of molten 1% low-melting agarose.

Physical form

Solution in 40 mM Tris-HCl, pH 7.5, 50 mM NaCl and 50% glycerol (v/v)

WGK Germany

3

T Imai et al.
Genomics, 8(2), 297-303 (1990-10-01)
We describe an improved method for construction of yeast artificial-chromosome (YAC) libraries that contain large inserts of foreign DNA. The procedure consists of seven steps: (i) preparation of human DNA in agarose beads; (ii) partial digestion of the DNA with...

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