A9414

Sigma-Aldrich

Agarose, low gelling temperature

BioReagent, for molecular biology

Synonym(s):
2-Hydroxyethyl agarose
CAS Number:
MDL number:
NACRES:
NA.25

biological source

algae (red)

Quality Level

grade

for molecular biology

product line

BioReagent

form

powder

EEO

≤0.10

mp

≤65 °C

transition temp

congealing temperature 26-30 °C

gel strength

≥200 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.10%

foreign activity

DNase, RNase, NICKase, none detected

Related Categories

General description

Agarose is a component of cell wall polysaccharides of marine red algae. It has high gelling property and is hydrophilic. It exists in random coil conformation.

Application

Agarose, low gelling temperature has been used:
  • in the single cell gel electrophoresis of glioblastoma multiforme cells for genotoxicity studies
  • in the preparation of collagen-agarose co-gels
  • in the embedding of shrimp brain sections

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

Biochem/physiol Actions

Agarose is used in enzyme immobilization and electrophoresis. It is also used in food and cosmetic industry.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Insect-like organization of the stomatopod central complex: functional and phylogenetic implications
Thoen HH, et al.
Frontiers in Behavioral Neuroscience, 11(7), 12-12 (2017)
Mechanical and structural contribution of non-fibrillar matrix in uniaxial tension: a collagen-agarose co-gel model
Lake SP and Barocas VH
Annals of Biomedical Engineering, 39(7), 1891-1903 (2011)
Wioletta Skronska-Wasek et al.
American journal of respiratory and critical care medicine, 196(2), 172-185 (2017-03-01)
Chronic obstructive pulmonary disease (COPD), in particular emphysema, is characterized by loss of parenchymal alveolar tissue and impaired tissue repair. Wingless and INT-1 (WNT)/β-catenin signaling is reduced in COPD; however, the mechanisms thereof, specifically the role of the frizzled (FZD)...
Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastoma multiforme cells
Hinzmann M, et al.
International journal of nanomedicine, 9(1), 2409-2409 (2014)
A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
L Wieslander
Analytical biochemistry, 98(2), 305-309 (1979-10-01)

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