All Photos(3)

B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):
Bluo-Gal, 5-Bromo-3-indolyl β-D-galactopyranoside
Empirical Formula (Hill Notation):
C14H16BrNO6
CAS Number:
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

assay

≥98% (HPLC)

form

powder

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

InChI key

LINMATFDVHBYOS-MBJXGIAVSA-N

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General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Packaging

100, 500 mg in poly bottle

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Leslie M Stone et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(11), 4522-4529 (2002-06-01)
Taste buds are a heterogeneous population of cells exhibiting diverse morphological and biochemical characteristics. Because taste buds arise from multiple progenitors, the different types of taste cells may represent distinct lineages. The present study was undertaken to determine the following:
Cardiac origin of smooth muscle cells in the inflow tract
Nakano H, et al.
Journal of Molecular and Cellular Cardiology, 50(2), 337-345 (2011)
Matthew M Goddeeris et al.
Development (Cambridge, England), 134(8), 1593-1604 (2007-03-09)
Cardiac outflow tract (OFT) septation is crucial to the formation of the aortic and pulmonary arteries. Defects in the formation of the OFT can result in serious congenital heart defects. Two cell populations, the anterior heart field (AHF) and cardiac
Delineation of matriptase protein expression by enzymatic gene trapping suggests diverging roles in barrier function, hair formation, and squamous cell carcinogenesis
List K, et al.
The American Journal of Pathology, 168(5), 1513-1525 (2006)
Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination
Kirsten JH, et al.
Developmental Biology, 287(1), 146-156 (2005)

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