D4913

Sigma-Aldrich

2′-Deoxycytidine 5′-triphosphate disodium salt

100 mM (pH 7)

Synonym(s):
dCTP-Na2
Empirical Formula (Hill Notation):
C9H14N3Na2O13P3
CAS Number:
Molecular Weight:
511.12
MDL number:
PubChem Substance ID:
NACRES:
NA.52

assay

≥99%

Quality Level

form

liquid

concentration

100 mM (pH 7)

color

colorless

foreign activity

DNase, RNase, none detected

shipped in

dry ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2

InChI

1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1

InChI key

ABWVCNMFYVEBIB-CDNBRZBRSA-L

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General description

Deoxycytidine triphosphate (dCTP) is a cytidine analog.

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used in reverse transcription polymerase chain reaction (RT -PCR).
2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Packaging

25 μmol in poly bottle
0.1 mmol in poly bottle

Biochem/physiol Actions

dCTP is utilized by cells for DNA polymerase catalyzed synthesis of DNA.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Nicole Bertoletti et al.
Protein science : a publication of the Protein Society, 28(9), 1664-1675 (2019-07-14)
The retrovirus HIV-1 has been a major health issue since its discovery in the early 80s. In 2017, over 37 million people were infected with HIV-1, of which 1.8 million were new infections that year. Currently, the most successful treatment...
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, C-C (1989)
Mingyue He et al.
Methods in molecular biology (Clifton, N.J.), 484, 193-205 (2008-07-02)
Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody-ribosome-mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by...
Marco Gatti et al.
Cell reports, 32(5), 107985-107985 (2020-08-07)
PARP inhibitors (PARPi) cause synthetic lethality in BRCA-deficient tumors. Whether specific vulnerabilities to PARPi exist beyond BRCA mutations and related defects in homology-directed repair (HDR) is not well understood. Here, we identify the ubiquitin E3 ligase TRIP12 as negative regulator...

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