H0268

Sigma-Aldrich

Hexanucleotide Primers

lyophilized powder

Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

storage temp.

−20°C

Other Notes

Hexanucleotide primers are a mixture of random 5′-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template.

Unit Definition

1 A260 unit = approx. 20 μg

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Methods for non-radioactive labeling of nucleic acids.
Kessler, C. et al.
Nonisotopic Probing, Blotting, and Sequencing, 51-54 (1995)
S Bauer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(5), 1792-1803 (2003-03-12)
The mammalian olfactory epithelium (OE) is composed of primary olfactory sensory neurons (OSNs) that are renewed throughout adulthood by local, restricted neuronal progenitor cells. The molecular signals that control this neurogenesis in vivo are unknown. Using olfactory bulb ablation (OBX)...
Bernhard Reuss et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(16), 6404-6412 (2003-07-25)
Multiple evidence suggests that fibroblast growth factors (FGFs), most prominently FGF-2, affect astroglial proliferation, maturation, and transition to a reactive phenotype in vitro, and after exogenous administration, in vivo. Whether this reflects a physiological role of endogenous FGF is unknown....
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
A P Feinberg et al.
Analytical biochemistry, 132(1), 6-13 (1983-07-01)
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I...

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