I0140

Sigma-Aldrich

Anti-Human IgM (μ-chain specific) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

application(s)

indirect ELISA: 1:100,000

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

IgM is a class of antibodies containing a pentamer of five IgG equivalents with 10 Fab fragments, joined by a single cross-linking peptide (J-chain). It constitutes 15% of the normal adult immunoglobulins.

Specificity

The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa, and lambda myeloma proteins.

Application

Anti-Human IgM (μ-chain specific) antibody produced in rabbit was used as secondary antibody in immunoblotting and indirect ELISA.

Biochem/physiol Actions

IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM is an initial immunoglobulin produced in the developing fetus as a result of the immune response during the second trimester of pregnancy. It is then substituted by IgG. Therefore, the diagnosis of specific antibodies of the IgM class is useful in detecting chronic infections. IgM is essential for the fetal and neonatal host defense system. IgM cannot cross the placenta. Hence, the presence of IgM might indicate an intrauterine viral infection. IgM antibodies are responsible for the agglutination of red blood cells in mismatched blood transfusions. The level of IgM may vary with the status of disease or infection.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Overview of the Immune Response
The Journal of Allergy and Clinical Immunology null
Immunology of the Fetus and Newborn
Avery's Diseases of the Newborn, 445-467 null
J Raper et al.
Infection and immunity, 67(4), 1910-1916 (1999-03-20)
Natural resistance of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in human serum of nonimmune factors that lyse the parasite. Normal human serum contains two trypanosome lytic factors (TLFs). TLF1 is a 500-kDa...
E J Wiersma et al.
Journal of immunology (Baltimore, Md. : 1950), 160(12), 5979-5989 (1998-06-24)
Previous studies have discerned two forms of polymeric mouse IgM: moderately cytolytic (complement-activating) pentamer, which contains J chain, and highly cytolytic hexamer, which lacks J chain. To investigate the relationships among polymeric structure, J chain content, and cytolytic activity, we...

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