IgM is a class of antibodies containing a pentamer of five IgG equivalents with 10 Fab fragments, joined by a single cross-linking peptide (J-chain). It constitutes 15% of the normal adult immunoglobulins.
The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones kappa, and lambda myeloma proteins.
Anti-Human IgM (μ-chain specific) antibody produced in rabbit was used as secondary antibody in immunoblotting and indirect ELISA.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM is an initial immunoglobulin produced in the developing fetus as a result of the immune response during the second trimester of pregnancy. It is then substituted by IgG. Therefore, the diagnosis of specific antibodies of the IgM class is useful in detecting chronic infections. IgM is essential for the fetal and neonatal host defense system. IgM cannot cross the placenta. Hence, the presence of IgM might indicate an intrauterine viral infection. IgM antibodies are responsible for the agglutination of red blood cells in mismatched blood transfusions. The level of IgM may vary with the status of disease or infection.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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