I6760

Sigma-Aldrich

Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse

clone HP-6017, ascites fluid

Synonym(s):
Anti-Human Fc Monoclonal Antibody, Anti-Human Fc Monoclonal Antibody - Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, Monoclonal Anti-Human IgG (Fc specific), Anti Human Igg Monoclonal Antibody, Mouse Anti-Human Igg
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6017, monoclonal

contains

15 mM sodium azide

species reactivity

sheep, horse (IgG), rabbit, goat, human

application(s)

indirect ELISA: 1:3,000

isotype

IgG2a

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family and have two heavy (H) and two light (L) chain, held together by disulphide linkages. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Specificity

The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • imprint immunofixation (IIF)
  • immunofluorometric assay (IFMA)
  • hemagglutination (HA)
  • hemagglutination inhibition (HAI)
  • particle counting immunoassay(PACIA)

Packaging

0.2, 0.5 mL in glass bottle

Biochem/physiol Actions

Although the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess a "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37-60

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Ratio of serum IgG3 to total IgG concentration and goiter size are independent factors in intractability of Graves' disease
Santoh T, et al.
Endocrine Journal, 0711080013-0711080013 (2007)
Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin
Vossenaar ER, et al.
Arthritis Research & Therapy, 6(2), R142-R142 (2004)
Effector-attenuating substitutions that maintain antibody stability and reduce toxicity in mice
Lo M, et al.
The Journal of Biological Chemistry, 292(9), 3900-3908 (2017)
Structure and function of immunoglobulins
Schroeder Jr HW and Cavacini L
The Journal of Allergy and Clinical Immunology, 125, S41-S52 (2010)
Maria L Balmer et al.
Science translational medicine, 6(237), 237ra66-237ra66 (2014-05-23)
A prerequisite for establishment of mutualism between the host and the microbial community that inhabits the large intestine is the stringent mucosal compartmentalization of microorganisms. Microbe-loaded dendritic cells trafficking through lymphatics are arrested at the mesenteric lymph nodes, which constitute...

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