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Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


for molecular biology

product line





suitable for nucleic acid staining

storage temp.

room temp

SMILES string




InChI key


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General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.


Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.


25, 100 g in glass bottle

Features and Benefits

  • Less hazardous than staining with ethidium bromide
  • Viewable in visible light
  • Fast and easy to use


Exclamation mark

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

13 - Non Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Young-Sharp, D. and Kumar, R.
Technique, 1, 183-183 (1989)
Angela L Davis et al.
International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)
Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of...
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
A C Peacock et al.
Biochemistry, 6(6), 1818-1827 (1967-06-01)
Fei Yan et al.
Photochemistry and photobiology, 86(3), 662-666 (2010-02-06)
This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also...
Alexandra Vaccaro et al.
PloS one, 7(7), e42117-e42117 (2012-08-01)
The DNA/RNA-binding proteins TDP-43 and FUS are found in protein aggregates in a growing number of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and related dementia, but little is known about the neurotoxic mechanisms. We have generated Caenorhabditis elegans and...

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