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M8194

Sigma-Aldrich

Anti-MAFF (C-Terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-v-maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family, ProteinF
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 18 kDa

species reactivity

human

application(s)

immunoprecipitation (IP): 2-4 μL using A431 cell line
microarray: suitable
western blot: 1:2,000-1:4,000 using whole extracts of the A431 cell line

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAFF(23764)

General description

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) is a transcription factor which does not have a transactivation domain but possesses a leucine-zipper.
The MAFF gene is located on the human chromosome at 22q13.1. The encoded protein is a small MAF protein and is expressed in the nucleus.

Specificity

Anti-MAFF (C-terminal) specifically recognizes MAFF.

Immunogen

synthetic peptide corresponding to amino acids 147-163 of human MAFF (isoform a). The immunizing peptide does not cross-react with other members of the family.

Application

Anti-MAFF (c-terminal) antibody produced in rabbit has been used in:
  • western blotting
  • chromatin immunoprecipitation (chip)
  • co-immunoprecipitation
  • immunoprecipitation

Biochem/physiol Actions

The small MAF proteins are associated with many physiological processes including development, differentiation, hematopoiesis and stress response. Interestingly, these proteins regulate the stress response via different mechanisms. The MAFF gene is down-regulated in diabetes mellitus 1 patient. This gene is underexpressed in acute lymphoblastic lymphoma (ALL) patients.
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) has been shown to interact with other transcription factors and it associates with certain regulatory sequences in the gene encoding the oxytocin receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Meenakshi B Kannan et al.
Biochimica et biophysica acta, 1823(10), 1841-1846 (2012-06-23)
The small MAFs, MAFF, MAFG and MAFK have emerged as crucial regulators of mammalian gene expression. Previous studies have linked small MAF function, by virtue of their heterodimerization with the Cap 'n' Collar (CNC) family of transcription factors, to the...
Yaeko Takagi et al.
Biochemical and biophysical research communications, 320(1), 62-69 (2004-06-23)
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to...
Julie A Moran et al.
The Biochemical journal, 361(Pt 2), 371-377 (2002-01-05)
The three small Maf proteins, MafF, MafG and MafK, have been implicated in a number of physiological processes, including development, differentiation, haematopoiesis and stress response. Here we report the constitutive expression of mafF, mafG and mafK in six human cell...
O Johnsen et al.
Nucleic acids research, 24(21), 4289-4297 (1996-11-01)
The human TCF11 gene encodes a ubiquitously expressed bZIP transcription factor of the cap n' collar (CNC) domain family. It has a high sequence similarity to the erythroid-specific bZIP factor p45 NF-E2 in the CNC domain, which is involved in...
Xiaoxia Ye et al.
Archives of biochemistry and biophysics, 449(1-2), 87-93 (2006-03-22)
The human transcription factor MafF (hMafF) lacks a transactivation domain, it contains a heptad-leucine repeat motif (viz., Leu-zipper) that may mediate protein-protein interactions to regulate transcriptional activities. A protein with a coiled-coil domain encoded by a novel human gene (GenBank...

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