MilliporeSigma
All Photos(1)

Documents

P5899

Sigma-Aldrich

Poly-L-lysine hydrobromide

mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

All Photos(1)
Synonym(s):
PDL HBr
CAS Number:
25988-63-0
MDL number:
MFCD00237230
eCl@ss:
32160406
PubChem Substance ID:
24898718

biological source

synthetic (organic)

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P6282P6407P4832
Poly-L-lysine hydrobromide mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

P5899

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

P6282

Poly-L-lysine hydrobromide

Poly-D-lysine hydrobromide mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Sigma-Aldrich

P6407

Poly-D-lysine hydrobromide

Sigma-Aldrich

Sigma-Aldrich

P4832

Poly-L-lysine solution

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

sterility

sterile-filtered

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

solution

mol wt

≥300,000

mol wt

70,000-150,000

mol wt

70,000-150,000

mol wt

150,000-300,000

packaging

pkg of 5 mg

packaging

pkg of 5 mg

packaging

pkg of 10 X 5 mg, pkg of 5 mg

packaging

pkg of 50 mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Skye E Smith et al.
eNeuro, 7(4) (2020-08-02)
Aberrant migration of inhibitory interneurons can alter the formation of cortical circuitry and lead to severe neurologic disorders including epilepsy, autism, and schizophrenia. However, mechanisms involved in directing the migration of interneurons remain incompletely understood. Using a mouse model, we
Stuart B Mazzone et al.
Molecular neurobiology, 57(2), 949-963 (2019-10-21)
Bronchopulmonary sensory neurons are derived from the vagal sensory ganglia and are essential for monitoring the physical and chemical environment of the airways and lungs. Subtypes are heterogenous in their responsiveness to stimuli, phenotype, and developmental origin, but they collectively
Shuhei Ishikura et al.
Nucleic acids research, 48(19), 10848-10866 (2020-10-01)
Centromeres are genomic regions essential for faithful chromosome segregation. Transcription of noncoding RNA (ncRNA) at centromeres is important for their formation and functions. Here, we report the molecular mechanism by which the transcriptional regulator ZFAT controls the centromeric ncRNA transcription
View All Related Papers

Articles

Poly-lysine | Poly-D-Lysine | Poly-L-Lysine

Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service