BioXtra, ≥99% (titration)

Piperazine-N,N′-bis(2-ethanesulfonic acid), Piperazine-1,4-bis(2-ethanesulfonic acid), 1,4-Piperazinediethanesulfonic acid
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

product line



≥99% (titration)




Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.5% (as SO4)


≤0.5% loss on drying, 110°C

useful pH range

6.1 - 7.5

pKa (25 °C)



>300 °C (lit.)


1 M NaOH: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.2%

cation traces

Al: ≤0.005%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.005%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.1%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%


≤0.1 at 260 in 1 M NaOH at 0.5 M
≤0.1 at 280 in 1 M NaOH at 0.5 M

Featured Industry

Diagnostic Assay Manufacturing

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General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.


Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.


50, 250 g in poly bottle


Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.


Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Y Zhou et al.
The Journal of biological chemistry, 275(10), 6975-6979 (2000-03-04)
The poor stability of membrane proteins in detergent solution is one of the main technical barriers to their structural and functional characterization. Here we describe a solution to this problem for diacylglycerol kinase (DGK), an integral membrane protein from Escherichia...
Sambrook , J. and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 7-7 (2001)
S Haviernick et al.
Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy...
K E Loesser et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(11), 1477-1485 (1986-11-01)
We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia...
A Giraudel et al.
Biochemistry, 37(24), 8724-8734 (1998-06-24)
The dissociation and separation of the tubulin alpha- and beta-subunits have been achieved by binding alpha-subunits to an immunoadsorbent gel and selectively inducing release of free beta-subunits. The immunoadsorbent gel was prepared by coupling the monoclonal antibody YL1/2 to Sepharose...
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
Read More
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Read More

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